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human monocytic leukaemia reporter cell line thp1  (InvivoGen)


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    Structured Review

    InvivoGen human monocytic leukaemia reporter cell line thp1
    Human Monocytic Leukaemia Reporter Cell Line Thp1, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tlr4+md2+cd14/THP1-Dual+MD2-CD14-TLR4+Cells/pmc12248214-65-7-15
    Average 95 stars, based on 159 article reviews
    human monocytic leukaemia reporter cell line thp1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: Burkholderia mallei expresses a unique lipopolysaccharide mixture that is a potent activator of human Toll-like receptor 4 complexes.
    Article Snippet: MALDI-TOF spectrometry was also performed on an Applied Biosystems 4700 Proteomics Analyzer in the positive ion reflector mode. .. Human embryonic kidney 293 (HEK 293) cells transfected with human MD2-CD14 (HEK 293-hMD2-CD14; Invivogen), human TLR2/CD14 (HEK 293-hTLR2/CD14; Invivogen) or human TLR4/MD2-CD14 (HEK 293-hTLR4/MD2-CD14; Invivogen) were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% heat-inactivated (HI) fetal bovine serum (FBS), 10 mg ml-1 Blasticidin S and 50 mg ml-1 Hygromycin B (Invivogen). ..

    Article Title: Burkholderia mallei expresses a unique lipopolysaccharide mixture that is a potent activator of human Toll-like receptor 4 complexes
    Article Snippet: MALDI-TOF spectrometry was also performed on an Applied Biosystems 4700 Proteomics Analyzer in the positive ion reflector mode. .. Human embryonic kidney 293 (HEK 293) cells transfected with human MD2-CD14 (HEK 293-hMD2-CD14; Invivogen), human TLR2/CD14 (HEK 293-hTLR2/CD14; Invivogen) or human TLR4/MD2-CD14 (HEK 293-hTLR4/MD2-CD14; Invivogen) were maintained at 37°C with 5% CO 2 in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% heat-inactivated (HI) fetal bovine serum (FBS), 10 μg ml −1 Blasticidin S and 50 μg ml −1 Hygromycin B (Invivogen). ..

    Article Title: Bradyrhizobium Lipid A: Immunological Properties and Molecular Basis of Its Binding to the Myeloid Differentiation Protein-2/Toll-Like Receptor 4 Complex
    Article Snippet: .. HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen) was seeded into 96-well plate at the concentration of 1 × 10 5 cells/mL. .. 48 h after seeding the cells were transiently transfected through PolyFect Transfection Reagent (Qiagen) with a reaction mix containing 150 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 15 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).

    Article Title: The Vi Capsular Antigen of Salmonella enterica Serotype Typhi Reduces Toll-Like Receptor-Dependent Interleukin-8 Expression in the Intestinal Mucosa
    Article Snippet: .. HEK293 cells stably transfected with human TLR4/MD2/CD14 or human TLR5 were purchased from Invivogen. ..

    Article Title: Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models
    Article Snippet: Instrument was calibrated within 1000–3150 Da mass range, using peptide mixture (Peptide Calibration Standard, Bruker Daltonics). .. The HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen), was seeded into a 24-well plate at the concentration of 5 × 10 5 cells/mL. .. 48 h after seeding, the cells were transiently transfected through PolyFect transfection reagent (Qiagen) with a reaction mix containing 250 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 25 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).

    Article Title: Structure-Based Design and Synthesis of Lipid A Derivatives to Modulate Cytokine Responses.
    Article Snippet: Human embryonic kidney cells (HEK) 293T cells were grown in Dulbecco’s modified Eagle’s medium (ATCC) with L-glutamine (4 mM), glucose (4.5 g/L), and sodium bicarbonate (1.5 g/L) supplemented with penicillin (100 u/mL) / streptomycin (100 μg/mL; Mediatech), Normocin (100 μg/mL; InvivoGen), and FBS (10%). .. Stably transfected HEK 293T cells with human TLR4/MD2/CD14 were obtained from InvivoGen and grown in the same medium as for HEK 293T cells supplemented with the selective agents blasticidin (10 μg/mL; InvivoGen) and HygroGold (50 μg/mL; InvivoGen). ..

    Modification:

    Article Title: Burkholderia mallei expresses a unique lipopolysaccharide mixture that is a potent activator of human Toll-like receptor 4 complexes.
    Article Snippet: MALDI-TOF spectrometry was also performed on an Applied Biosystems 4700 Proteomics Analyzer in the positive ion reflector mode. .. Human embryonic kidney 293 (HEK 293) cells transfected with human MD2-CD14 (HEK 293-hMD2-CD14; Invivogen), human TLR2/CD14 (HEK 293-hTLR2/CD14; Invivogen) or human TLR4/MD2-CD14 (HEK 293-hTLR4/MD2-CD14; Invivogen) were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% heat-inactivated (HI) fetal bovine serum (FBS), 10 mg ml-1 Blasticidin S and 50 mg ml-1 Hygromycin B (Invivogen). ..

    Article Title: Burkholderia mallei expresses a unique lipopolysaccharide mixture that is a potent activator of human Toll-like receptor 4 complexes
    Article Snippet: MALDI-TOF spectrometry was also performed on an Applied Biosystems 4700 Proteomics Analyzer in the positive ion reflector mode. .. Human embryonic kidney 293 (HEK 293) cells transfected with human MD2-CD14 (HEK 293-hMD2-CD14; Invivogen), human TLR2/CD14 (HEK 293-hTLR2/CD14; Invivogen) or human TLR4/MD2-CD14 (HEK 293-hTLR4/MD2-CD14; Invivogen) were maintained at 37°C with 5% CO 2 in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% heat-inactivated (HI) fetal bovine serum (FBS), 10 μg ml −1 Blasticidin S and 50 μg ml −1 Hygromycin B (Invivogen). ..

    Stable Transfection:

    Article Title: Bradyrhizobium Lipid A: Immunological Properties and Molecular Basis of Its Binding to the Myeloid Differentiation Protein-2/Toll-Like Receptor 4 Complex
    Article Snippet: .. HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen) was seeded into 96-well plate at the concentration of 1 × 10 5 cells/mL. .. 48 h after seeding the cells were transiently transfected through PolyFect Transfection Reagent (Qiagen) with a reaction mix containing 150 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 15 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).

    Article Title: The Vi Capsular Antigen of Salmonella enterica Serotype Typhi Reduces Toll-Like Receptor-Dependent Interleukin-8 Expression in the Intestinal Mucosa
    Article Snippet: .. HEK293 cells stably transfected with human TLR4/MD2/CD14 or human TLR5 were purchased from Invivogen. ..

    Article Title: Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models
    Article Snippet: Instrument was calibrated within 1000–3150 Da mass range, using peptide mixture (Peptide Calibration Standard, Bruker Daltonics). .. The HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen), was seeded into a 24-well plate at the concentration of 5 × 10 5 cells/mL. .. 48 h after seeding, the cells were transiently transfected through PolyFect transfection reagent (Qiagen) with a reaction mix containing 250 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 25 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).

    Article Title: Structure-Based Design and Synthesis of Lipid A Derivatives to Modulate Cytokine Responses.
    Article Snippet: Human embryonic kidney cells (HEK) 293T cells were grown in Dulbecco’s modified Eagle’s medium (ATCC) with L-glutamine (4 mM), glucose (4.5 g/L), and sodium bicarbonate (1.5 g/L) supplemented with penicillin (100 u/mL) / streptomycin (100 μg/mL; Mediatech), Normocin (100 μg/mL; InvivoGen), and FBS (10%). .. Stably transfected HEK 293T cells with human TLR4/MD2/CD14 were obtained from InvivoGen and grown in the same medium as for HEK 293T cells supplemented with the selective agents blasticidin (10 μg/mL; InvivoGen) and HygroGold (50 μg/mL; InvivoGen). ..

    Concentration Assay:

    Article Title: Bradyrhizobium Lipid A: Immunological Properties and Molecular Basis of Its Binding to the Myeloid Differentiation Protein-2/Toll-Like Receptor 4 Complex
    Article Snippet: .. HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen) was seeded into 96-well plate at the concentration of 1 × 10 5 cells/mL. .. 48 h after seeding the cells were transiently transfected through PolyFect Transfection Reagent (Qiagen) with a reaction mix containing 150 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 15 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).

    Article Title: Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models
    Article Snippet: Instrument was calibrated within 1000–3150 Da mass range, using peptide mixture (Peptide Calibration Standard, Bruker Daltonics). .. The HEK293 cell line, stably transfected with human TLR4/MD2-CD14 (InvivoGen), was seeded into a 24-well plate at the concentration of 5 × 10 5 cells/mL. .. 48 h after seeding, the cells were transiently transfected through PolyFect transfection reagent (Qiagen) with a reaction mix containing 250 ng of Firefly luciferase reporter constructs, pGL3.ELAM.tk [harboring nuclear factor kappa B (NF-κB) promoter sequences], and 25 ng of Renilla luciferase reporter plasmid, pRLTK (as an internal control).



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